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Protein unfolding with a steric trap. The on of protein flop requires a fashion to drive unfolding, which is habitually accomplished by altering solution conditions on the way to favor the denatured state. This has the undesirable result that the molecular forces responsible exchange for configuring the polypeptide chain are to boot changed. It would therefore be informative to develop routines that can move unfolding without the need for destabilizing solvent conditions.

At this point we introduce a new method just before accomplish this aim, which we request steric trapping. All the rage the steric trick method, the butt protein is labeled with two biotin tags placed niggardly in space accordingly that both biotin tags can sole be bound past streptavidin when the protein unfolds.

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